MATLAB Format Images ==================== Previous versions of CellProfiler supported reading and writing of MATLAB format (.mat) images. MATLAB format images were useful for exporting illumination correction functions generated by **CorrectIlluminationCalculate**. These images could be loaded and applied to other pipelines using **CorrectIlluminationApply**. This version of CellProfiler no longer supports reading or exporting MATLAB format images. Instead, the recommended image format for illumination correction functions is NumPy (.npy). Loading MATLAB format images can only be done in CellProfiler versions through 3.1.9. To ensure compatibility with future versions of CellProfiler you can convert your .mat files to .npy files via **SaveImages** using any 3.X build of CellProfiler. See **SaveImages** for more details on saving NumPy format images.